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- Leonard Schuele1,*
, Leandre Murhula Masirika2,3,4,*, Jean Claude Udahemuka5,6,*, Freddy Belesi Siangoli7, Justin Bengehya Mbiribindi7, Pacifique Ndishimye6,8, Frank M Aarestrup9, Marion Koopmans1, Bas B Oude Munnink1, Richard Molenkamp1, GREATLIFE MPOX group10 View AffiliationsHide Affiliations
Affiliations:
1Department of Viroscience, Erasmus University Medical Center, Rotterdam, the Netherlands
2Centre de Recherche en Sciences Naturelles de Lwiro, South Kivu, DS Bukavu, Democratic Republic of the Congo
3
4Congo Outbreaks, Research for Development, South Kivu, Bukavu, Democratic Republic of the Congo
5Department of Veterinary Medicine, University of Rwanda, Nyagatare, Rwanda
6Genomics Research and Development Division, Stansile Research Organization, Kigali, Rwanda
7Division Provinciale de la Santé, South Kivu, Bukavu, Democratic Republic of the Congo
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9Research Group for Genomic Epidemiology, National Food Institute, Technical University of Denmark, Kgs. Lyngby, Denmark
10The members of the network are listed under Collaborators
*These authors contributed equally to this work and share first authorship
Correspondence:
Leonard Schuele
L.schuleerasmusmc.nl
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Collaborators:Marjan Boter,Hayley Cassidy,Léandre Mutimbwa Mambo,Gitta Overbeek,Jean Pierre Musabyimana,Jules Ndoli,Saria Otani
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Citation style for this article:Schuele Leonard, Masirika Leandre Murhula, Udahemuka Jean Claude, Siangoli Freddy Belesi, Mbiribindi Justin Bengehya, Ndishimye Pacifique, Aarestrup Frank M, Koopmans Marion, Oude Munnink Bas B, Molenkamp Richard, GREATLIFE MPOX group.Real-time PCR assay to detect the novel Clade Ib monkeypox virus, September 2023 to May 2024.Euro Surveill. 2024;29(32):pii=2400486.https://doi.org/10.2807/1560-7917.ES.2024.29.32.2400486Received: 23 Jul 2024;Accepted: 07 Aug 2024
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Abstract
Monkeypox virus (MPXV) is an emerging zoonotic pathogen with complex epidemiology necessitating rapid diagnosis and distinguishing between clades and subclades. The emerging Clade Ib lacks the genomic region used in the Clade I-specific assay from the Centers for Disease Control and Prevention. We report an MPXV real-time PCR to specifically detect Clade Ib. The assay demonstrated proficient sensitivity and specificity in 92 samples and can be included along other TaqMan-based assays to detect MPXV and distinguish between clades and subclades.
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This work is licensed under a Creative Commons Attribution 4.0 International License.
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Real-time PCR assay to detect the novel Clade Ib monkeypox virus, September 2023 to May 2024
<p class="citation"><span>Citation style for this article:</span><span class="meta-value authors"><a href="/search?value1=Leonard+Schuele&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Schuele Leonard</a>, <a href="/search?value1=Leandre+Murhula+Masirika&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Masirika Leandre Murhula</a>, <a href="/search?value1=Jean+Claude+Udahemuka&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Udahemuka Jean Claude</a>, <a href="/search?value1=Freddy+Belesi+Siangoli&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Siangoli Freddy Belesi</a>, <a href="/search?value1=Justin+Bengehya+Mbiribindi&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Mbiribindi Justin Bengehya</a>, <a href="/search?value1=Pacifique+Ndishimye&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ndishimye Pacifique</a>, <a href="/search?value1=Frank+M+Aarestrup&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Aarestrup Frank M</a>, <a href="/search?value1=Marion+Koopmans&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Koopmans Marion</a>, <a href="/search?value1=Bas+B+Oude+Munnink&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Oude Munnink Bas B</a>, <a href="/search?value1=Richard+Molenkamp&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Molenkamp Richard</a>, <a href="/search?value1=GREATLIFE+MPOX+group&option1=author&noRedirect=true" class="nonDisambigAuthorLink">GREATLIFE MPOX group</a></span>.Real-time PCR assay to detect the novel Clade Ib monkeypox virus, September 2023 to May 2024.<a href="/content/ecdc">Euro Surveill.</a> 2024;29(32):pii=2400486.<a href="https://doi.org/10.2807/1560-7917.ES.2024.29.32.2400486" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2024.29.32.2400486</a><span class="ES_Article_citation"><span class="generated">Received</span>: 23 Jul 2024; <span class="generated">Accepted</span>: 07 Aug 2024 </span></p>
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